rabbit anti leptin receptor polyclonal antibody  (Boster Bio)

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: MTT Assay, Control, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Immunofluorescence staining of leptin receptor in BME-UV1 bovine mammary epithelial cells. Panels I and II present the localization of leptin receptor detected using primary antibodies (cat. no. bs-0961; Bioss Antibodies), followed by secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); nuclei were counterstained with 7-amino actinomycin (7-AAD, red fluorescence). Panel III presents no primary antibody control. Micrographs were taken at 600× magnification. Scale bar: 20 μm.

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: Immunofluorescence, Staining, Fluorescence, Control

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. ( A ) Representative dot-plots of Annexin V/PI double staining in untreated control cells; ( B ) graph showing percentage of apoptotic cells (sum of Annexin V pos /PI neg and Annexin V pos /Pi pos cells) in control and adipokine-treated cells; ( C ) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; ( D , E ) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: Double Staining, Control, Western Blot, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Concentration of αS1-casein in BME-UV1 cells ( A ) and media ( B ) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Untreated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance ( p < 0.05).

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: Concentration Assay, Negative Control, Positive Control, Standard Deviation, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 μm.

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: Immunofluorescence, Staining, Cell Culture, Control, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software ( https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: Cell Culture, Control, Software, Standard Deviation, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells

doi: 10.3390/ijms25084147

Figure Lengend Snippet: List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).

Article Snippet: Leptin receptor Rabbit Polyclonal Antibody , Bioss Antibodies , bs-0961R , 1:200 , IF.

Techniques: Staining

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: LEPR expression in CD8 + T cells from vitiligo patients was increased. (A, B) Double immunofluorescence staining results of LEPR and CD8 in vitiligo lesions and normal skin (Vitiligo: N = 5, NC: N = 5). (A) CD8 was represented by green fluorescence and LEPR was represented by red fluorescence. (B) The expression of LEPR in CD8 + T cells in Vitiligo lesions was significantly higher. (C) The serum levels of leptin were evaluated by ELISA (Vitiligo: N=53, HC: N=36). (D, E) Representative flow cytometric profiles and data plots of CD8 + T cells in PBMCs are shown (Vitiligo: N=5, HC: N=5). * P < 0.05, ** P < 0.01.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Expressing, Double Immunofluorescence Staining, Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: Leptin enhances the expression of cytotoxic cytokines from CD8 + T cells in vitro . (A, B) Gating strategy, representative flow cytometric plots, and statistical analysis of the percentage of cell subsets were shown. (C) After 72 hours of leptin stimulation, the protein levels of IFN-γ, perforin, and Granzyme B from normal PBMCs were detected by ELISA. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Expressing, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Leptin deficiency in CD8 + T cells ameliorates non-segmental vitiligo by reducing interferon-γ and Granzyme B

doi: 10.3389/fimmu.2023.1158883

Figure Lengend Snippet: Leptin deficiency in CD8 + T cells ameliorated vitiligo development in mice. (A) The hair decolorization areas of mice with vitiligo induced by monobenzone are shown, marked with red circles. (B) HE staining showed that the inflammatory cells of the Lep KO mice were significantly reduced compared with the WT group. (C) The heatmap shows the differential expression of vitiligo-related genes between WT and Lep KO mice after monobenzone was administrated. (D) RT-qPCR was used to verify the differentially expressed genes in CD8 + T cells from spleens of vitiligo mice induced by monobenzone in the Lep KO group and WT group. * P < 0.05, *** P < 0.001.

Article Snippet: The following antibodies were used: Alexa Flour 647 Donkey anti-rabbit IgG (BioLegend), Rabbit Anti-Leptin receptor antibody (bs-0410R, Bioss), APC-Cy7 anti-human CD4 (BD Biosciences), Percp-Cy5.5 anti-human CD8 (BD Biosciences), PE anti-human Perforin (BD Biosciences), FITC anti-human/mouse Granzyme B (Granzyme B, BioLegend), PE-Cy7 anti-human IFN-γ (BioLegend), Zombie Aqua Fixable Viability Kit (BioLegend), APC-Cy7 anti-mouse CD4 (Abcam), Percp-Cy5.5 anti-mouse CD8 (Abcam), PE anti-mouse Perforin (Abcam), FITC anti-mouse/human granzyme B (BioLegend), PE-Cy7 anti-mouse IFN-γ (Abcam).

Techniques: Staining, Expressing, Quantitative RT-PCR